Y1771
Yeast Synthetic Drop-out Medium Supplements
without uracil, leucine, and tryptophan
Synonyme(s) :
Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements
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form
powder
technique(s)
transformation: suitable
application(s)
microbiology
storage temp.
room temp
Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:
- the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
- Yarrowia lipolytica PO1f strain positive transformants
- CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants
Preparation Note
Dissolve 1.46 g of supplement per liter of medium.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
Contenu apparenté
Instructions
Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| Y1771-20G | 04061837587979 |