S8015
SSC Buffer
for Northern and Southern blotting, powder blend
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NACRES:
NA.25
UNSPSC Code:
41105319
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Molecular Biology
form
powder blend
pH
6.9-7.1 (20-25 °C, 20 ×)
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.
Application
Suitable for use in washing buffer for Northern and Southern hybridization procedures.
Features and Benefits
- Easy to reconstitute powder
- Multi-purpose use in hybridizations
Preparation Note
SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).
Classe de stockage
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
A J Bogers et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2(3), 160-166 (1988-01-01)
To identify risk factors for hospital mortality after postinfarction left ventricular aneurysmectomy (PILVA), 83 consecutive cases of PILVA were studied in a retrospective analysis. Hospital mortality was 14.4%. Most of the factors correlated with hospital mortality were associated with poor
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| S8015-1L | 04061836960490 |