S2015
SSPE Buffer 20× Concentrate
for Northern and Southern blotting, solution
Synonyme(s) :
20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
41105319
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Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
solution
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.
Application
Suitable for:
- Hybridization buffer for Northern and Southern blots
- Washing buffer for microarrays
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Preparation Note
SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
Pierce SE, et al.
Nature Protocols, 2, 2958-2974 (2007)
Glucocorticoid antagonist RU38486 fails to block acid-induced muscle wasting in vivo or in vitro.
Pickering WP, et al.
Nephrology, Dialysis, and Transplantation, 18, 1475-1484 (2003)
Warren P Pickering et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 18(8), 1475-1484 (2003-08-05)
Increased protein degradation during metabolic acidosis contributes to muscle wasting in uraemia. Adrenalectomy experiments in severely acidotic rats (arterial pH approximately 7.15) have shown that this is prevented in the absence of glucocorticoid. It should therefore be possible to block
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| S2015-1L | 04061836916527 |