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A propos de cet article
NACRES:
NA.31
UNSPSC Code:
41105300
Service technique
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pack of 1 ea
manufacturer/tradename
Cytiva RPN3004
storage temp.
2-8°C
General description
The world’s first commercially available chemiluminescent detection reagent for Western blotting. Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.
Based on the enhanced chemiluminescent reaction of luminol with horseradish peroxidase, ECL substrate can be used to detect probes which have been labelled either directly or indirectly with horseradish peroxidase. Rapid light output enables results to be achieved in 10 to 15 minutes. It is the substrate of choice for target amounts above 500 fg.
High sensitivity and fast results:
Based on the enhanced chemiluminescent reaction of luminol with horseradish peroxidase, ECL substrate can be used to detect probes which have been labelled either directly or indirectly with horseradish peroxidase. Rapid light output enables results to be achieved in 10 to 15 minutes. It is the substrate of choice for target amounts above 500 fg.
High sensitivity and fast results:
- Detects up to 10 to 12 ng of protein, which is ~10× more than colorimetric methods
- Optimized to use with Amersham Protran nitrocellulose (NC) membranes
- Delivers results in just a few minutes, then image using a CCD imager like the Amersham ImageQuant 800 or Amersham Hyperfilm ECL
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ECL is a trademark of Cytiva
Classe de stockage
12 - Non Combustible Liquids
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Articles
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
C Couturier et al.
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Type II secreted phospholipase A(2) (sPLA(2)) releases precursors of important inflammatory lipid mediators from phospholipids. Some observations have indicated that the sPLA(2), which has been implicated in chronic inflammatory conditions such as arthritis, contributes to atherosclerosis in the arterial wall.
G Y Akita et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 5(2), 154-158 (1993-04-01)
A previously described bluetongue virus (BTV) serogroup polymerase chain reaction (PCR) assay was applied to clinical samples. The sensitivity of the BTV serogroup PCR was increased by the use of non-radioactive chemiluminescent hybridization. Unfractionated whole blood samples from rams experimentally
C Couturier et al.
The Journal of biological chemistry, 274(33), 23085-23093 (1999-08-07)
Type II-secreted phospholipase A(2) (type II-sPLA(2)) is expressed in smooth muscle cells during atherosclerosis or in response to interleukin-1beta. The present study shows that the induction of type II-sPLA(2) gene by interleukin-1beta requires activation of the NFkappaB pathway and cytosolic
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| GERPN3004 | 04061834085164 |