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56496

Calcéine AM

Name: Calcéine AM
Brand: SIGMA

Small Package (20 X 50 μg ), ≥90.0% (HPLC)

Synonyme(s) :

O,O′-Diacétate tétrakis(acétoxyméthyl) de calcéine, ester

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A propos de cet article

Formule empirique (notation de Hill) :

C₄₆H₄₆N₂O₂₃

Numéro CAS:

148504-34-1

Poids moléculaire :

994.86

NACRES:

NA.32

PubChem Substance ID:

329758218

UNSPSC Code:

12171500

MDL number:

MFCD05861516

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Propriétés

Quality Level

100

assay

≥90.0% (HPLC)

fluorescence

λ_ex 496 nm; λ_em 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca²⁺

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

Description

General description

Calcein-AM, also known as Calcein-acetoxymethyl ester, is a non-fluorescent molecule converted into an anionic fluorescent form by intracellular esterase enzymes. It provides a continuous visualization of adherent cells during the experiment. Calcein-AM is a vital dye that introduces Calcein in living cells with intact cell membranes. The fluorescent part of Calcein-AM localizes in the intracellular spaces after esterase-dependent cellular trapping, displaying cytotoxic activity in cells.

Application

Dérivé de calcéine non fluorescent et perméable dans les cellules, devient fluorescent après hydrolyse. Utilisé comme substrat neutre de la protéine de multirésistance aux médicaments MRP ; utilisé dans la recherche sur le cancer.

Calcein-AM is used as a cell viability stain and as a neutral substrate for multidrug (MDR) efflux transporters. In cancer research, it is used as a neutral substrate for multidrug resistance protein MRP. Fluorescence assays to determine human erythrocyte viability and aging uses Calcein-AM as a probe.

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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Numéro d'article de commerce international

Référence	GTIN
56496-20X50UG	04061826687901