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MilliporeSigma

12106C

Sérum de veau fœtal

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonyme(s) :

FBS, FCS, sérum, sérums

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A propos de cet article

NACRES:
NA.71
MDL number:
UNSPSC Code:
12352207
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
Service technique
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biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Application

Fetal Bovine Serum (FBS) provides many non-defined growth promoting and survival enhancing factors to cells in culture. FBS, heat inactivated may be used in place of regular FBS wherein the inactivation of the complement system is beneficial to cell growth or function. Heat-inactivated FBS is used to culture Leishmania sp.

Preparation Note

Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Taux d′endotoxines et d′hémoglobine testés
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions



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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Protocoles

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

Voir tous les protocoles

E Anitua et al.
Experimental eye research, 119, 27-34 (2013-12-19)
We have developed and characterized a new type of plasma rich in growth factors (PRGF) derived eye-drop therapy for patients suffering from autoimmune diseases. To determine the concentration of several growth factors, proteins, immunoglobulins and complement activity of the heat-inactivated
Nand Lal Sharma et al.
The American journal of tropical medicine and hygiene, 72(6), 819-824 (2005-06-21)
Localized cutaneous leishmaniasis (LCL) in India is due mostly to Leishmania tropica. It is mainly endemic in the deserts of Rajasthan. Recently, Himachal Pradesh has been identified as a new endemic focus for the disease. In the last few years
Li Zhou et al.
Genome biology, 9(7), R119-R119 (2008-07-31)
CD8+ NKT-like cells are naturally occurring but rare T cells that express both T cell and natural killer cell markers. These cells may play key roles in establishing tolerance to self-antigens; however, their mechanism of action and molecular profiles are



Numéro d'article de commerce international

RéférenceGTIN
12106C-1L04065266965339
12106C-1000ML04061838717528
12106C-100ML04061838717702
12106C-500ML04061838717719