Chlorhydrate de Trizma^®

Tris HCl, or Trizma^® hydrochloride, serves as a widely utilized acidic buffer with a pKa of 8.08 at 25°C, offering a pH range of 7.0 to 9.0, aligning with the typical pH of living organisms. It is a crucial component in buffer solutions for diverse biological, cell biology, and biochemistry applications. Notably, Tris base and Tris hydrochloride individually lack sufficient buffering capacity, necessitating their combination to create effective buffers within the pH range of 7 to 9.

Essential in protein electrophoresis and western blotting techniques, Tris buffers, particularly low ionic strength versions, are employed for protein transfer. They are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, integral to DNA agarose electrophoresis, contribute to buffer preparations like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA), playing pivotal roles in biochemistry, protein purification, and electrophoresis applications. Overall, Tris HCl proves to be a versatile and indispensable component, ensuring optimal pH in biological systems for effective biochemical and cell biology research.

Le pH de tous les tampons dépend de la température et de la concentration. Pour les tampons Tris, le pH augmente d'environ 0,03 unité par °C de diminution de la température et diminue de 0,03-0,05 unité par dilution d'un facteur 10. Pour les applications précises, utiliser un pH-mètre soigneusement calibré avec une électrode de verre au calomel.

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The pH values of all buffers are temperature and concentration-dependent. For Tris buffers, pH increases by about 0.03 unit per °C decrease in temperature and decreases by 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany