PPB011
Solution saline tamponnée au Tris
powder, pH 7.6, pHast Pack™
Synonyme(s) :
TBS
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Solution saline tamponnée au Tris, pH 7.6, pHast Pack™, powder
product line
pHast Pack™
Quality Level
form
powder
storage condition
(Tightly closed. Dry)
impurities
≤5 ppm Heavy Metals (lead)
color
white
pH
7.6
solubility
soluble, clear, colorless
cation traces
Fe: ~10 ppm
foreign activity
DNase, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNase, none detected
storage temp.
room temp
General description
Tris Buffered Saline, or TBS, pH 7.6 is a commonly used wash buffer that is isotonic and non-toxic and ideal for use in cell culture and electrophoresis applications.
Application
Tris-buffered saline can be used as a wash buffer for:
- Enzyme Linked Immunosorbent assay (ELISA)
- Western Blot and other protein applications
- Suitable for use in cell culture (recommend to sterile filter)
- Immunohistochemistry
- Sample preparation
- Resuspension and sonication of cells
Features and Benefits
- Ready for use, just dissolve in ultrapure water
- No calculations, measuring, and pH adjustments needed
- Save time and minimize effort
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm
Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl, pH 7.6 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture applications.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
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Contenu apparenté
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| PPB011-20PAK | 04061842323210 |