PPB001
Solution saline tamponnée au Tris
powder, pH 8.0, pHast Pack™
Synonyme(s) :
TBS
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Solution saline tamponnée au Tris, pH 8.0, pHast Pack™, powder
product line
pHast Pack™
form
powder
storage condition
(Tightly closed. Dry)
impurities
≤5 ppm Heavy Metals (lead)
color
white
pH
8.0
solubility
soluble, clear, colorless
cation traces
Fe: <10 ppm
foreign activity
DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected
storage temp.
room temp
General description
Tris Buffered Saline, pH 8.0 is a commonly used wash buffer that is isotonic and nontoxic and suitable for use in cell culture and electrophoresis applications.
Application
Tris-buffered saline is intended to use for:
- Sample preparation
- ELISA
- Western Blot and other protein applications
- Cell culture (recommend to sterile filter)
- Immunohistochemistry
- Resuspension and sonication of cells
Features and Benefits
- Ready-to-use buffer – fast and easy to prep
- No need to measure or adjust pH, simply add water
- Saves time and minimizes the effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 50mM Tris, 138mM NaCl, 2.7mM KCl, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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