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11383221001

Roche

BCIP

solution, >95% (HPLC), pkg of 3 mL (150 mg)

Synonyme(s) :

4-toluidine salt

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UNSPSC Code:
12352204
NACRES:
NA.54
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description

4-toluidine salt

assay

>95% (HPLC)

form

solution

packaging

pkg of 3 mL (150 mg)

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

General description

BCIP serves as a substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Application

Use BCIP in alkaline phosphatase detection:
  • Southern blot
  • Northern blot
  • Western blot
  • Colony and plaque hybridization
  • In situ hybridization

Biochem/physiol Actions

Use of BCIP as substrate for alkaline phosphatase in combination with nitro blue tetrazolium chloride (NBT) as electron acceptor: Substrates and reaction products of alkaline phosphatase catalyzed color reaction with NBT/BCIP.

Physical form

Solution (50 mg/ml) in dimethylformamide (DMF)

Analysis Note

Function test: incubation with AP

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

Supply conditions do not apply to the regions and states of Brazil: North, Northeast, Mato Grosso do Sul, Mato Grosso, and Rio Grande do Sul.

signalword

Danger

Hazard Classifications

Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Repr. 1B

Classe de stockage

3 - Flammable liquids

wgk

WGK 2

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Certificats d'analyse (COA)

Lot/Batch Number
16853420
13513023
13513022
59318426
59318425

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Consulter la Bibliothèque de documents

Gregor Eichele et al.
Methods (San Diego, Calif.), 53(4), 417-423 (2010-12-28)
Genome-scale sequencing projects, high-throughput RNAi screens, systematic gene targeting, and system-biology-based network predictions all depend on a validation of biological significance in order to understand the relevance of a particular finding. Such validation, for the most part, rests on low-throughput
Nicoletta I Petridou et al.
Cell, 184(7), 1914-1928 (2021-03-18)
Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural
Ewa Pocheć et al.
Acta biochimica Polonica, 62(2), 323-328 (2015-06-23)
Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes
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RéférenceGTIN
1138322100104061838701497