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MilliporeSigma

MABE967

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1

clone 11E10.1, from mouse

Synonyme(s) :

DNA polymerase delta catalytic subunit, DNA polymerase delta p125, DNA polymerase subunit delta p125

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
11E10.1, monoclonal
Application:
WB
Citations:
-
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11E10.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... POLD1(5424)

General description

DNA polymerase delta catalytic subunit (EC 2.7.7.7; UniProt P28340; also known as CDC2 homolog, DNA polymerase subunit delta p125) is encoded by the POLD1 (also known as CDC2, MDPL, POLD, CRCS10) gene (Gene ID 5424) in human. DNA replication in eukaryotes involves multiple enzymes including the B family polymerases Pol α, Pol δ, and Pol ɛ. While Pol α initiates DNA synthesis, Pol δ and Pol ɛ replace Pol α after primer extension and perform the bulk of DNA replication. Pol δ and Pol ɛ are heterotetramers in higher eukaryotes comprised of POLD1 and POLE as the respective catalytic subunit, as well as accessory subunits (POLD2/3/4 and POLE2/3/4) that interact with cofactors such as Proliferating Cell Nuclear Antigen (PCNA). Both POLD1 and POLE contain an exonuclease domain (a.a. 268–471 of POLE and 304–517 of POLD1) that proof-reading capability during DNA replication. In addition to DNA synthesis, Pol ɛ and Pol δ are believed to participate in chromosomal DNA repairs, including nucleotide excision repair (NER), ismatch repair (MMR) and repair of double strand breaks (DSBR). Germline exonuclease domain mutations (EDMs) in POLD1 and POLE cause polymerase proofreading-associated polyposis (PPAP). Tumors with EDMs exhibits dramatically increased base substitutions.
~125 kDa observed.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of human DNA polymerase delta p125.

Application

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 is an antibody against DNA polymerase delta p125 Antibody for use in Western Blotting.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DNA polymerase delta p125 in 10 µg of Ramos cell lysate.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA polymerase delta p125 in 10 µg of Raji cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.


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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Contenu apparenté

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Numéro d'article de commerce international

RéférenceGTIN
MABE96704055977294743