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MilliporeSigma

ESG1107

Protéine LIF de souris recombinante ESGRO®

ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.

Synonyme(s) :

Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF

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A propos de cet article

UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32160801
Form:
liquid
Assay:
>95% (active component mLIF, SDS-PAGE)
Service technique
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Quality Level

assay

>95% (active component mLIF, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

10000000 units/mL

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

mouse ... LIF(16878)

General description

Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Application



SUGGESTED PROTOCOLS:

ES Cells: Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).

Physical form

Format : purifié

Preparation Note

®
A cold pack may be included for customer relations, however it is truly not needed. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. ° Freeze-thawing will reduce potency. ° Freeze-thawing should be avoided. °

Analysis Note

Activité spécifique: ®& μ

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay:

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany


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12 - Non Combustible Liquids



Certificats d'analyse (COA)

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Protocoles

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Articles

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Naive pluripotent stem cells cultured in vitro using specialized media and inhibitors mimic "ground-state" cells from blastocysts.

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Contenu apparenté

"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."

Voir tous les contenus connexes

Gesa Witt et al.
Cell biology and toxicology, 37(2), 229-243 (2020-06-22)
The embryonic stem cell test (EST) represents the only validated and accepted in vitro system for the detection and classification of compounds according to their developmental and reproductive teratogenic potency. The widespread implementation of the EST, however, in particular for
Xudong Fu et al.
Science advances, 6(22), eaay5181-eaay5181 (2020-06-12)
In mouse embryonic stem cell (ESC), a small cell population displays totipotent features by expressing a set of genes that are transiently active in 2-cell-stage embryos. These 2-cell-like (2C-like) cells spontaneously transit back into the pluripotent state. We previously dissected
Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
Janebodin, Kajohnkiart, et al.
Testing, 6, e27526-e27526 (2011)



Numéro d'article de commerce international

RéférenceGTIN
ESG110708436037124310