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362185

PNGase F Glycosidase

Name: PNGase F Glycosidase
Brand: MM

Removes all N-linked glycans, suitable for mass spectrometry, from Elizabethkingia meningosepticum

Synonyme(s) :

N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F

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A propos de cet article

Numéro CAS:

83534-39-8

Numéro CE :

3.5.1.52 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.54

MDL number:

MFCD00131222

Specific activity:

≥20,000 units/mg protein, ≥4500 units/mL

Biological source:

bacterial (Elizabethkingia meningosepticum)

Service technique

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Propriétés

Nom du produit

N-Glycosidase F, Elizabethkingia meningosepticum,

biological source

bacterial (Elizabethkingia meningosepticum)

Quality Level

100

conjugate

(N-linked)

form

liquid

specific activity

≥20,000 units/mg protein, ≥4500 units/mL

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Other Notes

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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flash_point_f

Not applicable

flash_point_c