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MilliporeSigma

1.25193

Cellvento® 4HEK Liquid

Chemically defined, sterile; sterile-filtered, suitable (for HEK293 cell culture)

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UNSPSC Code:
12352200
Service technique
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Quality Level

description

shipped with cooling pack.

Liquid medium must be supplemented with 6–10 mM L-Glutamine.

sterility

sterile; sterile-filtered

product line

Cellvento®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

storage temp.

2-8°C

General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

Cellvento® 4HEK Medium can be used as amplification and production medium for gene therapy applications. This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines. Cellvento®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages. This easy-to-use medium can be used for all stages like growth, transfection, and production.

Preparation Note

Formulated to contain 30.8 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany


Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

The current status and future manufacturing of AAV-based gene therapies is discussed in this podcast transcript, including how to streamline large-scale manufacturing.

Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.

Minimize the complexities of cell and gene therapy production. Switch to upstream viral vector platforms to enhance scalability and simplify GMP manufacturing.

Voir tous les articles

Contenu apparenté

A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.

Solution pour la production de vecteurs viraux basée sur la transfection, utilisant une lignée cellulaire en suspension, un milieu chimiquement défini et un procédé éprouvé à l'échelle réelle.





Numéro d'article de commerce international

RéférenceGTIN
125193100004065266964325