RDRT
ReadyScript® cDNA Synthesis Mix
Complete reagent for first strand cDNA synthesis for RT-qPCR
Synonym(s):
cDNA Synthesis Mix
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About This Item
usage
sufficient for 100 reactions, sufficient for 25 reactions, sufficient for 500 reactions
feature
dNTPs included, hotstart: no
technique(s)
RT-PCR: suitable, cDNA synthesis: suitable
color
colorless
input
purified RNA
shipped in
dry ice
storage temp.
−20°C
General description
Application
Features and Benefits
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
- Includes proprietary blend of randomers and oligodT primers and an RNAse H+ derivative of Moloney murine leukemia virus (MMLV) reverse transcriptase
- Allows for unbiased, accurate conversion of RNA to cDNA in just 40 minutes
- Can use up to 1 μg of RNA in a single 20 μL reaction; reactions can also be scaled up
- Highly stable; can be stored at -20°C for up to 1 year; 4°C for up to one month
- Specific ratio of randomers and oligo (dT) primers in the mastermix ensures optimal representation of all transcript sequences in the cDNA product
- High reproducibility: Premixed master mix eliminates variability inherent in other kits requiring mixing of components
- RNase H(+) Moloney murine leukemia virus (MMLV) reverse transcriptase ensures residual RNA does not interfere with PCR amplification
- Reverse transcribes both rare and abundant genes with a wide input range of template
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
Other Notes
Legal Information
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
Articles
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
Related Content
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
La réaction en chaîne par polymérase (PCR pour "Polymerase Chain Reaction") est une technique d'amplification des molécules d'acide nucléique qui est couramment employée dans de nombreuses applications, incluant la RT-PCR, la PCR à démarrage à chaud, la PCR en point final, et bien d'autres.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| RDRT-500RXN | 04061835514014 |
| RDRT-100RXN | 04061835514007 |
| RDRT-25RXN | 04061835574834 |