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QPBCA

QuantiPro™ BCA Assay Kit

for 0.5-30 μg/ml protein

Synonym(s):

BCA Assay, Protein determination by BCA, bicinchoninic acid reagent

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Application

In addition to protein determination and quantification in solution, the QuantiPro™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Biochem/physiol Actions

Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.

Features and Benefits

  • Accurate across a broad range of protein concentrations
  • High sensitivity; linear response form 0.5 to 30 μg/ml of protein
  • Stable color complex
  • Reduced susceptibility to detergents

Preparation Note

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Legal Information

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • P0914
    Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1
    SDS

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Articles

Western Blot Analysis of Immunoprecipitation (IP-Western)

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Cell Lysate Preparation

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

Universal Proteomics Standards

High-throughput proteomics advances with improved analysis methods and mass spectrometry.

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Global Trade Item Number

SKUGTIN
QPBCA-1KT04061835573943