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PP0200

GlycoProfile™ I, In-Gel Deglycosylation Kit

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About This Item

UNSPSC Code:
12352200
EC Number:
232-615-7
NACRES:
NA.32
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shelf life

≥1 yr at 2‑8 °C

Quality Level

200

storage temp.

2-8°C

Application

The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Biochem/physiol Actions

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Preparation Note

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • P7367
    PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U
    SDS
  • T6567
    Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg
    SDS
  • Trypsin Solubilization Reagent
    SDS
  • I0408
    Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae
    SDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

35.6 °F

flash_point_c

2.0 °C

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
037K6810
016K6837
065K6251
023K9176

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Articles

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

View All Articles

Global Trade Item Number

SKUGTIN
PP0200-1KT04061836686888