KAPA2G Robust HotStart PCR Kit

The second-generation KAPA2G Robust DNA Polymerase evolves to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

KAPA2G Robust HotStart^® PCR Kit has been used for:

• Amplification of GC- or AT-rich templates
• Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
• Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
• Colony PCR
• qPCR
• Nested polymerase chain reaction (PCR) amplification
• Amplification of DNA targets in PCR
• DNA amplification using conventional PCR
• Routine PCR

DNA fragments created with KAPA2G Robust HotStart^® DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase. It can be used for routine downstream applications, such as restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Robust HotStart has 5′→3′ polymerase and 5′→3′ exonuclease activities, devoid of 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Robust HotStart is similar to wild-type Taq. It shows an error rate of approximately 1 error per 1.7 x 10⁵ nucleotides incorporated.

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

Each batch of KAPA2G Robust HotStart^® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Robust HotStart PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.