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MilliporeSigma

Jurkat E6.1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88042803, human blood (leukemic T-cell lymphoblast), Not specified

Synonym(s):

Jurkat Cells, Jurkat Clone E6-1 Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

human blood (leukemic T-cell lymphoblast)

packaging

tube of 5 μg 88042803-DNA-5UG
pkg of vial of cells 88042803-1VL

growth mode

Suspension

karyotype

Pseudodiploid, modal No 46

morphology

Not specified

products

Interleukin 2 (IL-2), human alpha interferon

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
  • study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
  • determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
  • study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

Biochem/physiol Actions

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
Human leukaemic T cell lymphoblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

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Articles

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Protocols

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

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