HSANGERG
Sanger Arrayed Whole Genome Lentiviral CRISPR Library
Human, Glycerol Format
Synonym(s):
Sanger CRISPR Library
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About This Item
UNSPSC Code:
41105904
Quality Level
packaging
pkg of 50 μL (96-well plate)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.
Application
Functional Genomics/Screening /Target Validation
Features and Benefits
- Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
- Simplify the workflow with puromycin selection
- Illuminate CRISPR-expressing cells with BFP
- Flip the expression components into and out of the genome using transposase
Additional Features
- Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
- Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
- Collaborative: Real-time, library validation continues
For detailed information on the Sanger library, click here
Packaging
96-well plates
Physical form
50 μl of bacterial glycerol stocks, 383×96-well plates
Other Notes
Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones
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Request a Quote or More Information
This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
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Protocols
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
생어 시퀀싱 단계 또는 연쇄 종결 방법과 DNA 시퀀싱의 작동 방식, 연구를 위해 생어 시퀀싱 결과를 정확하게 판독하는 방법에 대해 알아보세요.
Articles
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al.
Scientific Reports, 7 (2017)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| HSANGERG-1EA | 04061826266199 |