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MilliporeSigma

G004027

HPLC Analysis of Warfarin Enantiomers on Astec® CYCLOBOND I 2000 (UV at 254 nm)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CYCLOBOND I 2000, 25 cm × 4.6 mm I.D., 5 μm particles (20024AST)
mobile phase: [A] acetonitrile: [B] acetic acid: [C] triethylamine; (100:0.3:0.25, A:B:C)
flow rate: 1 mL/min
column temp.: 23 °C
detector: UV, 254 nm
injection: 5 μL

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

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Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

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