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MilliporeSigma

G002340

HPLC Analysis of Warfarin Anticoagulant on Ascentis® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 20 mM phosphoric acid (pH 2.0, unadjusted): [B] methanol; (30:70, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 10 μL
sample: 50 μg/mL each in mobile phase

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
    Warfarin

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