66825-U
BIOshell™ A400 Protein C4 (3.4 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Synonym(s):
Protein C4 Reversed-Phase Chromatography Column
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About This Item
Product Name
BIOshell™ A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel hardware
Quality Level
agency
suitable for USP L26
description
Shell thickness (0.2 μm), Solid Core (3.0 μm)
product line
BIOshell™
feature
endcapped
manufacturer/tradename
BIOshell™
packaging
1 ea of
extent of labeling
0.4% carbon loading
parameter
600 bar max. pressure, 90 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
15 m2/g
surface coverage
4.2 μmol/m2
matrix
spherical silica particle platform, superficially porous particle
matrix active group
C4 (butyl) phase
particle size
3.4 μm
pore size
400 Å
operating pH range
2-9
separation technique
reversed phase
General description
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Protocols
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Articles
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.
Related Content
La HPLC separa e identifica biomoléculas grandes como proteínas y péptidos mediante el bombeo de la muestra a través de una columna rellena de adsorbente.
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 66825-U | 04061833388143 |