WGA2
GenomePlex® Complete Whole Genome Amplification (WGA) Kit
Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
Synonym(s):
Whole genome amplification kit
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About This Item
UNSPSC Code:
41121800
NACRES:
NA.55
Related Categories
General description
GenomePlex® Complete Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It contains everything required for whole genome amplification including an optimized enzyme, WGA DNA Polymerase. It can be used in several applications and is suitable for use with purified genomic DNA from a variety of sources including blood cards, whole blood, buccal swabs, soil, plant, and serum. It allows the researcher to generate a representative, ~500-fold amplification of genomic DNA.
Application
GenomePlex® Complete Whole Genome Amplification (WGA) Kit has been used in the amplification of DNA. This kit is also suitable for use with downstream applications including:
- microarray analysis
- SNP analysis
- STR analysis
- DNA archiving
Features and Benefits
- Higher yield from minimal template: Amplification of nanogram amounts of genomic DNA to microgram yields (around 10 μg) in less than about three hoursrs
- Nanograms of samples can be preserved at –20 °C for future use
- Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)
- Whole-genome amplification (WGA) DNA polymerase increases the amplification accuracy
- Whole-genome representation with no detectable allele bias
- Compatible with many downstream applications such as TaqMan® assays, single nucleotide polymorphism (SNP) analysis, comparative genomic hybridization (CGH) analysis
Other Notes
The sequences of the universal primers provided in this kit are considered proprietary.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
Kit Components Also Available Separately
Product No.
Description
SDS
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Levi Yant et al.
The Plant cell, 22(7), 2156-2170 (2010-08-03)
The Arabidopsis thaliana transcription factor APETALA2 (AP2) has numerous functions, including roles in seed development, stem cell maintenance, and specification of floral organ identity. To understand the relationship between these different roles, we mapped direct targets of AP2 on a
M Korabecna et al.
Neoplasma, 63(3), 402-410 (2016-03-02)
Tubulocystic renal cell carcinoma (TRCC) represents a rare tumor with incidence lower than 1 % of all renal carcinomas. This study was undertaken to contribute to characterization of molecular signatures associated with TRCC and to compare them with the features of papillary
Tao Chen et al.
Toxicology, 292(2-3), 63-70 (2011-11-15)
Furan, a widely used industrial compound, has been found in a number of heated food items. Furan is carcinogenic to rats and mice, but the mechanism behind its carcinogenic effect is still not well understood. In this study, we tested
Karolina Åberg et al.
European journal of human genetics : EJHG, 20(9), 953-955 (2012-03-02)
DNA from Epstein-Barr virus-transformed lymphocyte cell lines (LCLs) has proven useful for studies of genetic sequence polymorphisms. Whether LCL DNA is suitable for methylation studies is less clear. We conduct a genome-wide methylation investigation using an array set with 45
Zhong Zhao et al.
Nature, 465(7301), 1089-1092 (2010-06-26)
The classic phytohormones cytokinin and auxin play essential roles in the maintenance of stem-cell systems embedded in shoot and root meristems, and exhibit complex functional interactions. Here we show that the activity of both hormones directly converges on the promoters
Protocols
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Protocol extracts genomic DNA from blood cards, useful for limited DNA samples in amplification.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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