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MilliporeSigma

T6414

Sigma-Aldrich

Trypsin inhibitor from Glycine max (soybean)

solution, sterile-filtered, suitable for, suitable for cell culture

Synonym(s):

SBTI

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About This Item

CAS Number:
9035-81-8
MDL number:
MFCD00082103
UNSPSC Code:
12352204
NACRES:
NA.75

Key Documents

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biological source

Glycine max (soybean)

Quality Level

100

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

20,100 Da

concentration

10 mg/mL

technique(s)

cell culture | mammalian: suitable

solubility

balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble

suitability

suitable for

UniProt accession no.

P01070

shipped in

dry ice

storage temp.

−20°C

Gene Information

Glycine max (soybean) ... KTI3(547831)

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Application

Optimized for passage of endothelial cell cultures.
Trypsin inhibitor from Glycine max (soybean) was used for the following applications:
  • Filteration and seeding of dispersed acini
  • Relative quantification of phospho-tau and immunoblotting analysis
  • Subcloning and transformation of VIL-4 and IL-4 cDNA, and protein expression and purification (trypsin inhibitor form glycine max is used as positive control).

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Preparation Note

Prepared in Dulbecco′s Phosphate Buffered Saline without calcium and magnesium.
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.

Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Other Notes

Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
View more information on Trypsin Inhibitor.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK5594
SLCH2926
SLCG7585
SLCD9017
SLCB6865

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ZhuQing Yuan et al.
Journal of immunology (Baltimore, Md. : 1950), 182(2), 811-819 (2009-01-07)
The possibility that mycobacterial infections induce variant cytokine mRNA encoding a functionally distinct protein for immune regulation has not been addressed. In this study, we reported that Mycobacterium tuberculosis and bacillus Calmette-Guérin infections of macaques induced expression of variant IL-4
Doubly Phosphorylated Peptide Vaccines to Protect Transgenic P301S Mice against Alzheimer's Disease Like Tau Aggregation.
Monique Richter et al.
Vaccine, 2(3), 601-623 (2014)
Johann Gout et al.
Journal of visualized experiments : JoVE, (78)(78), doi:10-doi:10 (2013-08-28)
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 10(6) acinar cells can be obtained from a single
Zhiyong Mi et al.
Cancers, 15(12) (2023-06-28)
Cancer progression depends on an accumulation of metastasis-supporting physiological changes, which are regulated by cell-signaling molecules. In this regard, a disintegrin and metalloproteinase 8 (Adam8) is a transmembrane glycoprotein that is selectively expressed and induced by a variety of inflammatory
Deepti Ramachandran et al.
Scientific reports, 8(1), 10818-10818 (2018-07-19)
Studies indicate that modulating enterocyte metabolism might affect whole body glucose homeostasis and the development of diet-induced obesity (DIO). We tested whether enhancing enterocyte fatty acid oxidation (FAO) could protect mice from DIO and impaired glycemic control. To this end
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