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T6146

Trypan Blue

Name: Trypan Blue
Brand: SIGMA

powder, BioReagent, suitable for cell culture

Synonym(s):

Direct blue 14, Niagara blue reagent, blue live/dead cell assay

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About This Item

Linear Formula:

C₃₄H₂₄N₆O₁₄S₄Na₄

CAS Number:

72-57-1

Molecular Weight:

960.81

EC Number:

200-786-7

UNSPSC Code:

12352207

NACRES:

NA.75

PubChem Substance ID:

57654686

MDL number:

MFCD00003969

Beilstein/REAXYS Number:

4360496

Colour Index Number:

23850

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Properties

product line

BioReagent

Quality Level

300

form

powder

composition

Dye content, ~40%

packaging

glass bottle of 100 g, glass bottle of 25 g, glass bottle of 5 g

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

mp

>300 °C (lit.)

solubility

H₂O: 10 mg/mL

application(s)

cell analysis

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

human ... PTPN1(5770), PTPRF(5792)

Description

General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Application

Trypan blue has been used:

in cell viability assay
to stain plant tissue in order to to visualize cell death

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Preparation Note

To make a cell viability solution, add the T6146 Trypan Blue powder to a clean and dry glass or plastic container of appropriate capacity. Add a suitable solvent, such as sterile distilled water, to the container with the Trypan Blue powder. Stir the solution vigorously using a stir bar or a glass rod until the powder has completely dissolved. A 0.4% solution is a common concentration for typical cell viability assessment. For example, to make 100 mL of a 0.4% solution, add 400 milligrams (0.4 g) to 99.6 mL solvent.

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pictograms

GHS08

signalword

Danger

hcodes

H350

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number

23796BJ

25496TH

09614EH

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Protocols

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3D Spheroid BME Cell Invasion Assay utilizes specialized plates and ECM for cell aggregation and spheroid formation in vitro.

Articles

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Global Trade Item Number

SKU	GTIN
T6146-100G	04061835569632
T6146-25G	04061835569649
T6146-5G	04061835569656