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R7020

Transcript RNA Markers 0.2-10 kb

Name: Transcript RNA Markers 0.2-10 kb
Brand: SIGMA

for RNA electrophoresis

Synonym(s):

RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105337

Form:

liquid

Solubility:

water: soluble

Storage temp.:

−70°C

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Properties

grade

Molecular Biology

Quality Level

300

form

liquid

usage

25 uses

solubility

water: soluble

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

Description

General description

Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides

For optimal resolution, the recommended agarose gel concentration is 1.0%.

Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Global Trade Item Number

SKU	GTIN
R7020-50UG	04061836695972