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MilliporeSigma

R4142

RNA Marker template set

~0.5 mg/mL in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA

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About This Item

UNSPSC Code:
12352200
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grade

Molecular Biology

concentration

~0.5 mg/mL in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA

storage temp.

−20°C

Application

In vitro transcription with T7 RNA polymerase produces transcripts 100, 200, 300, 400, 600, 800 and 1,000 bases long. The transcripts can be labeled by including biotinylated, radioactive, or other labeled rNTP′s in the reaction. The markers are useful for RNase protection assays or for electrophoresis of low molecular weight RNA fragments.

Other Notes

A mixture of seven linearized plasmids for use as templates for RNA markers.


Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1



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