P3563
Phosphate Buffered Saline
powder, pH 7.4, with 0.05% Tween® 20
Synonym(s):
1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
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About This Item
UNSPSC Code:
41105319
eCl@ss:
32129211
NACRES:
NA.25
Product Name
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,
form
powder
Quality Level
color
white
solubility
water: soluble, clear, colorless (1 pouch dissolved in 1L)
application(s)
cell analysis
diagnostic assay manufacturing
General description
Phosphate buffered saline with TWEEN® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.
Application
Phosphate buffered saline has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA)
- telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
- cell isolation
Packaging
Foil pouches
Preparation Note
Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
TWEEN is a registered trademark of Croda International PLC
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma
Liu Z, et al.
Oncology Letters, 11(3), 1829-1834 (2016)
Shorter telomeres in luminal B, HER-2 and triple-negative breast cancer subtypes
Heaphy C M, et al.
Modern Pathology, 24(2), 194-194 (2011)
Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P3563-5X10PAK | 04061835547548 |
| P3563-10PAK | 04061835547531 |
| P3563-1EA | 04061824081541 |