MIRRT
MystiCq® microRNA cDNA Synthesis Mix
Synonym(s):
microRNA cDNA Synthesis Mix
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About This Item
NACRES:
NA.55
UNSPSC Code:
12352200
technique(s)
cDNA synthesis: suitable
shipped in
wet ice
storage temp.
−20°C
General description
Starting with total RNA or samples pre-enriched or microRNAs, the MystiCq® microRNA cDNA Synthesis Mix kit contains all the components necessary to convert mature microRNAs into cDNA templates for qPCR. Since microRNAs are not naturally polyadenylated, the first step in the cDNA synthesis process is to polyadenylate the microRNAs through a poly(A) polymerase reaction. Next, the ReadyScript reverse transcriptase, an oligo-dT adapter primer and other required reagents are added to convert the polyadenylated microRNAs into cDNA. The adapter primer includes a unique sequence at the 5′ end that is complementary to the sequence of the MystiCq Universal PCR Primer, another component of the MystiCq microRNA qPCR Assay System.The kit also contains a Positive Control Primer that is specific to SNORD44, a small nucleolar RNA that is widely expressed in most human tissues. Sufficient amounts of the Poly(A) Tailing Buffer and the MicroRNA cDNA Reaction mix are included in the kit to allow for the use of no poly(a) polymerase and no reverse transcriptase control reactions. To analyze individual microRNAs, use the MystiCq Universal PCR Primer (which targets the adapter primer sequence) together with the MystiCq microRNA qPCR Assay Primer and the MystiCq® microRNA SYBR® Green qPCR ReadyMix™.
Application
MystiCq® microRNA cDNA Synthesis Mix has been used for first-strand cDNA synthesis by reverse-transcription of miRNAs for quantitative polymerase chain reaction (qPCR) starting from total RNA
Features and Benefits
- Separate polyadenylation and reverse transcription steps allow for the possibility of optimization of protocol if necessary
- cDNA produced may be archived for future use
- Single RT reaction can provide up to 1000 qPCR reactions
- Includes a positive control primer for use with most human tissues
Packaging
Default reaction volume is 20 μL
Legal Information
MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
Kit Components Only
Product No.
Description
- Poly (A) Tailing Buffer (5X)
- Human Positive Control Primer
- Nuclease-free Water
- MystiCq® Universal PCR Primer
- Poly (A) Polymerase
- microRNA cDNA Reaction Mix
- ReadyScript® Reverse Transcriptase
Storage Class
10 - Combustible liquids
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Protocols
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
Articles
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
Quantitative PCR detection builds on basic PCR techniques and allows researchers to estimate the quantity of starting material in a sample with a much wider dynamic range.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MIRRT-25RXN | 04061835513895 |
| MIRRT-100RXN | 04061835574391 |