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M6018

4-Methylumbelliferyl β-D-cellobioside

glucanase substrate, fluorogenic, ≥98.00% (TLC), powder

Synonym(s):

4-Methylumbelliferyl beta-D-cellobioside

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About This Item

Empirical Formula (Hill Notation):
C22H28O13
CAS Number:
72626-61-0
Molecular Weight:
500.45
NACRES:
NA.32
PubChem Substance ID:
24897067
UNSPSC Code:
12352204
EC Number:
276-744-7
MDL number:
MFCD00063279
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Product Name

4-Methylumbelliferyl β-D-cellobioside, glucanase substrate

Quality Level

200

assay

≥98.00% (TLC)

form

powder

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-MKQZUAMYSA-N

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
  • to assay cellulase from A. niger
  • to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
  • to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
128K4069
128K4070
119H4117
116H4008
110K4105

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G P Xue et al.
Journal of general microbiology, 138(11), 2397-2403 (1992-11-01)
A plant polysaccharide hydrolase cDNA, designated celD, was isolated from a cDNA library of the rumen fungus Neocallimastix patriciarum. The enzyme encoded by celD had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that celD cDNA can be truncated to
Surface adhesion of fusion proteins containing the hydrophobins HFBI and HFBII from Trichoderma reesei
Linder M, et al.
Protein Science, 11(9), 2257-2266 (2002)
K H Jung et al.
Biochemistry and molecular biology international, 44(2), 283-292 (1998-04-08)
A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes
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Global Trade Item Number

SKUGTIN
M6018-100MG04061834060581
M6018-500MG04061834060598