M3770
Micrococcus lysodeikticus ATCC No. 4698
suitable for substrate for the assay of lysozyme, lyophilized cells
Synonym(s):
Micrococcus luetus
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
form
lyophilized cells
Quality Level
suitability
suitable for substrate for the assay of lysozyme
storage temp.
−20°C
Application
Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles.
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes.
Biochem/physiol Actions
Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.
Analysis Note
Contains polynucleotide phosphorylase.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Still not finding the right product?
Explore all of our products under Micrococcus lysodeikticus ATCC No. 4698
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold.
John D Steemson et al.
PloS one, 9(1), e86050-e86050 (2014-01-28)
The OB-fold is a small, versatile single-domain protein binding module that occurs in all forms of life, where it binds protein, carbohydrate, nucleic acid and small-molecule ligands. We have exploited this natural plasticity to engineer a new class of non-immunoglobulin
J C Cox et al.
Bioorganic & medicinal chemistry, 9(10), 2525-2531 (2001-09-15)
The in vitro selection of nucleic acid binding species (aptamers) is frequently repetitive, time-consuming, and poorly adapted to high-throughput applications. We have adapted automated workstations to select anti-protein aptamers; as an example, we demonstrated the selection of anti-lysozyme aptamers that
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M3770-25G | 04061834050582 |
| M3770-100G | 04061834050575 |
| M3770-5G | 04061835506415 |