Select a Size
About This Item
form
resin (dry)
crosslinking
cross-linked
mol wt
protein Mr 800-5000 Da (fractionation range), protein Mr 8000 Da (exclusion limit)
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0033-01
storage condition
dry media
technique(s)
liquid chromatography (LC): suitable
matrix
cross-linked dextran
particle size
50-150 μm (dry), 85-255 μm (wet)
cleaning in place
2-13
operating pH
2-13
separation technique
size exclusion (SEC)
storage temp.
2-30°C
General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).
Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Excellent recovery and minimum sample dilution
- Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
- BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications
Preparation Note
Analysis Note
Legal Information
Still not finding the right product?
Explore all of our products under Sephadex® G-25 Medium
Storage Class
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Articles
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Related Content
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-0033-01 | 04061834083344 |