D4812
REDAccuTaq® LA DNA Polymerase
High fidelity Taq with inert dye, 10X buffer included
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About This Item
Concentration:
1 unit/μL
Quality Level
form
liquid
usage
sufficient for 250 reactions
feature
Long & Accurate PCR, dNTPs included: no, hotstart: no
concentration
1 unit/μL
technique(s)
PCR: suitable
color
red
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.
REDAccuTaq® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3′→5′ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA.
Application
REDAccuTaq® LA DNA Polymerase produce long DNA amplicons for:
- Mutation analysis
- Cloning genes
- DNA sequencing
- cDNA library generation
- Amplicon sequencing
Features and Benefits
- Increased fidelity, up to 6.5× that of Taq DNA polymerase
- Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
- Visual confirmation that enzyme has been added, and proper mixing has occurred
- No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis
Packaging
The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.
Other Notes
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDAccuTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Protocols
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
Articles
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Sarah L Batt et al.
Journal of clinical microbiology, 43(6), 2656-2661 (2005-06-16)
Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping
Claudia Knief et al.
Applied and environmental microbiology, 71(7), 3826-3831 (2005-07-08)
Three upland soils from Thailand, a natural forest, a 16-year-old reforested site, and an agricultural field, were studied with regard to methane uptake and the community composition of methanotrophic bacteria (MB). The methane uptake rates were similar to rates described
Matthew J Ellis et al.
Breast cancer research and treatment, 119(2), 379-390 (2009-10-22)
Mutations in the alpha catalytic subunit of phosphoinositol-3-kinase (PIK3CA) occur in approximately 30% of ER positive breast cancers. We therefore sought to determine the impact of PIK3CA mutation on response to neoadjuvant endocrine therapy. Exons 9 (helical domain) and 20
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D4812-250UN | 04061833585702 |