D2532
Denhardt′s Solution 50x
for Northern and Southern blotting, solution
Synonym(s):
blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Quality Level
form
solution
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
General description
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Preparation Note
Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D2532-5X5ML | 04061838255143 |
| D2532-5ML | 04061838104885 |