C7902
Calcium chloride dihydrate
BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
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About This Item
Empirical Formula (Hill Notation):
CaCl2 · 2H2O
CAS Number:
Molecular Weight:
147.01
NACRES:
NA.71
PubChem Substance ID:
UNSPSC Code:
12352302
EC Number:
233-140-8
MDL number:
Assay:
≥99.0%
Form:
powder
Solubility:
H2O: 294 mg/mL, clear, colorless
product line
BioReagent
Quality Level
assay
≥99.0%
form
powder
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
mp
176 °C (dec.) (lit.)
solubility
H2O: 294 mg/mL, clear, colorless
SMILES string
O.O.Cl[Ca]Cl
InChI
1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
InChI key
LLSDKQJKOVVTOJ-UHFFFAOYSA-L
General description
Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl2 is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl2 is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl2 on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.
Application
Calcium chloride dihydrate has been used:
- in the formulation of media
- in sperm collection and preparation
- in cell culture and protoplast preparation
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C7902-500G | 04061835543540 |
| C7902-1KG | 04061835543533 |