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B9673

1-Bromo-3-chloropropane

Name: 1-Bromo-3-chloropropane
Brand: SIGMA

for isolation of RNA

Synonym(s):

1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide

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About This Item

Linear Formula:

Cl(CH₂)₃Br

CAS Number:

109-70-6

Molecular Weight:

157.44

UNSPSC Code:

41105515

NACRES:

NA.52

PubChem Substance ID:

24892149

EC Number:

203-697-1

Beilstein/REAXYS Number:

605278

MDL number:

MFCD00000998

Assay:

≥98.5% (GC)

Grade:

Molecular Biology

Bp:

144-145 °C (lit.)

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Properties

grade

Molecular Biology

Quality Level

200

assay

≥98.5% (GC)

form

liquid

refractive index

n20/D 1.486 (lit.)

bp

144-145 °C (lit.)

density

1.592 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

ClCCCBr

InChI

1S/C3H6BrCl/c4-2-1-3-5/h1-3H2

InChI key

MFESCIUQSIBMSM-UHFFFAOYSA-N

Description

General description

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

feline teeth
mice brain and liver tissues
human ectocervical epithelial cells
placental samples
human mesenchymal stromal cells (hMSCs)

Features and Benefits

less toxic alternative to chloroform
compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Other Notes

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.