B1911
BCIP®/NBT
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Product Name
BCIP®/NBT Liquid Substrate System, ready to use solution
Quality Level
form
liquid
storage temp.
2-8°C
Application
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
- for staining alkaline phosphatase(ALP) in bone marrow stromal cells
- as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
- as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B1911-100ML | 04061838251626 |