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A9941

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

Name: 2,2&#8242;-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
Brand: SIGMA

chromogenic, tablet

Synonym(s):

AzBTS-(NH₄)₂, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

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About This Item

Empirical Formula (Hill Notation):

C₁₈H₂₄N₆O₆S₄

CAS Number:

30931-67-0

Molecular Weight:

548.68

NACRES:

NA.21

PubChem Substance ID:

24891410

UNSPSC Code:

12352204

EC Number:

250-396-6

MDL number:

MFCD00010404

Beilstein/REAXYS Number:

7329461

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Properties

Product Name

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, tablet, 10 mg substrate per tablet

Quality Level

200

form

tablet

solubility

deionized water: 100 mL

storage temp.

room temp

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

Description

General description

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:

For a reaction with laccase or bilirubin oxidase
For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide

A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound

Application

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†
ABTS is also used to measure glucose concentrations in different solutions including blood serum.
The food industry uses ABTS to measure the antioxidant capacities within different foods.
Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Preparation Note

Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

wgk

WGK 2

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Protocols

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Global Trade Item Number

SKU	GTIN
A9941-4TAB	04061831168501
A9941-5TAB	04061833418314
A9941-100TAB	04061833418291
A9941-50TAB	04061833418307