Amido Black Staining Solution 2X

Name: Amido Black Staining Solution 2<SC>X</SC>
Brand: SIGMA

electrophoresis reagent

Synonym(s):

Naphthol Blue Black solution, Amido Black 10B

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About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₄N₆Na₂O₉S₂

CAS Number:

1064-48-8

Molecular Weight:

616.49

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

24891210

MDL number:

MFCD00004017

Beilstein/REAXYS Number:

5374263

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Properties

Quality Level

200

form

liquid

SMILES string

[Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

Description

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Application

Amido Black Staining Solution 2X has been used in the staining:

serum proteins present in the culture supernatant
cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
histones from chromatin samples

Preparation Note

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.