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MilliporeSigma

S0751

Sodium phosphate monobasic

ReagentPlus®, ≥99.0%

Synonym(s):

Monosodium dihydrogen orthophosphate, Monosodium phosphate, Sodium dihydrogen phosphate

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About This Item

Linear Formula:
NaH2PO4
CAS Number:
Molecular Weight:
119.98
PubChem Substance ID:
eCl@ss:
38070208
UNSPSC Code:
12161700
NACRES:
NA.21
EC Number:
231-449-2
MDL number:
Technical Service
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Quality Level

product line

ReagentPlus®

assay

≥99.0%

form

powder or crystals

technique(s)

DNA extraction: suitable, electrophoresis: suitable, immunocytochemistry: suitable

pH

4.0-4.5 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

water: 100 mg/mL, clear, colorless

application(s)

detection

SMILES string

[Na+].OP(O)([O-])=O

InChI

1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

AJPJDKMHJJGVTQ-UHFFFAOYSA-M

General description

Sodium phosphate monobasic acts as an acidulant in laxatives and effervescent powders. It is used in beverages, cheese and soft drink formulations.

Application

Sodium phosphate monobasic has been used:
  • as a reagent for virus transfection and fiber implantation in mouse
  • in phosphate buffer for immunocytochemistry and to detect individual catalase activity by PAGE (polyacrylamide gel electrophoresis)
  • as a buffer for cycling assay
  • in automated DNA extraction method
  • as a chemical in artificial cerebro-spinal fluid (aCSF) solution for the preparation of brainstem-spinal cord from rats

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC


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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Articles

Characterize mAb monomers, aggregates, and fragments using SEC-UV workflow with Zenix® and Zenix®-C SEC columns.


In vitro Brainstem-spinal Cord Preparation from Newborn Rat
Le Gal JP, et al.
The Journal of Neuroscience (2016)
Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD+) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry
Kanamori KS, et al.
Bio-protocol, 8(14) (2018)
Dictionary of Food ingredients (2001)



Global Trade Item Number

SKUGTIN
S0751-3KG04061835549078
S0751-500G04061835549085
S0751-100G04061835549054
S0751-1KG04061835549061