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About This Item
NACRES:
NA.21
UNSPSC Code:
41106300
Assay:
99.8% (sum of 4 peaks, HPLC)
Form:
solution
Solubility:
water: miscible
Storage temp.:
−20°C
grade
Molecular Biology
assay
99.8% (sum of 4 peaks, HPLC)
form
solution
usage
sufficient for 2,500 reactions (04638956001), sufficient for 5,000 reactions (11814362001), sufficient for 500 reactions (11581295001)
packaging
pkg of 10 × 200 μL (11814362001 [10mM, each]), pkg of 200 μL (11581295001 [10 mM, each]), pkg of 5 × 200 μL (04638956001 [10 mM, each])
manufacturer/tradename
Roche
concentration
10 mM
impurities
DNase, none detected, RNase, none detected
color
colorless
solubility
water: miscible
storage temp.
−20°C
General description
PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.
Application
- The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR
- RT-PCR
- cDNA synthesis
- Primer extension
Features and Benefits
The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.
Improve yield and performance
Improve yield and performance
- Choose a convenient ready-to-use mix of all 4 nucleotides.
- Achieve the highest PCR and RT-PCR sensitivity.
- Insist on highest purity (>99%) nucleotides.
Packaging
1 set containing 4 ready-to-use mixes
Preparation Note
Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.
Analysis Note
Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- dATP, PCR Grade, sodium salt 10 mM
- dCTP, PCR Grade, sodium salt 10 mM
- dGTP, PCR Grade, sodium salt 10 mM
- dTTP, PCR Grade, sodium salt 10 mM
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 4638956001 | 04061832355801 |
| 11581295001 | 04061837893469 |
| 11814362001 | 04061837893544 |