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MilliporeSigma

P5542

Sigma-Aldrich

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym(s):

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

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About This Item

CAS Number:
37288-19-0
EC Number:
4.6.1.13 (BRENDA, IUBMB)
EC Number:
232-638-2
MDL number:
MFCD00131896
UNSPSC Code:
12352204
NACRES:
NA.32

Key Documents

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form

lyophilized powder

Quality Level

300

specific activity

≥1,000 units/mg protein (Lowry)

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein

storage temp.

−20°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

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General description

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Other Notes

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
049M4179V
11190169
118M4150V
098M4076V
11180524

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Helen Neuert et al.
Development (Cambridge, England), 147(2) (2019-12-22)
The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily
Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
Brameshuber M
The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)
Danielle K Bailey et al.
PloS one, 16(7), e0254794-e0254794 (2021-07-27)
Iron and other transition metals, such as copper and manganese, are essential for supporting brain function, yet over-accumulation is cytotoxic. This over-accumulation of metals, particularly iron, is common to several neurological disorders; these include Alzheimer's disease, Parkinson's disease, Friedrich's ataxia
Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
Heinz DW
The Embo Journal, 14(16), 3855-3863 (1995)
Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells.
Sartori MJ
Tropical Medicine & International Health, 8(9), 832-839 (2003)
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Articles

Glycosylphosphatidylinisotol-anchored Glycoproteins Overview

Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.

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