Skip to Content
MilliporeSigma

NDEGLY

Sigma-Aldrich

Native Protein Deglycosylation Kit

Synonym(s):

Protein Deglycosylation Kit

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

conjugate

(N-linked)

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

All complex oligosaccharides can be reduced to the trimannosylchitobiose core by treatment of the glycoproteins with neuraminidase, β-galactosidase, and N-acetylglucosaminidase. Fucosidases may be required in some situations. The remaining trimannosylchitobiose core structures can be removed with endoglycosidase F3. Biantennary and triantennary structures can be immediately removed by endoglycosidases F2 and F3, respectively. Oligomannose and hybrid structures can be removed by Endoglycosidase F1.
For more information on each type of endoglycosidase, please refer to the Bulletin.
The Native Protein Deglycosylation is designed for the deglycoslylation of N-linked oligosaccharides from PNGase F-resistant native proteins. Endoglycosidases F1, F2, and F3 are less sensitive to protein conformation than PNGase F and are more suitable for removal of all classes of N-linked oligosaccharides without protein denaturation.

Application

Native Protein Deglycosylation Kit has been for N-deglycosylation of various enzymes such as laccase, tomato nuclease TBN1 and endo-β-1,3-glucanase.

Preparation Note

The NDEGLY Kit ships on wet ice and storage at 2–8 °C is recommended. This kit may be used for at least 1 year when stored as indicated. Several days exposure to ambient temperatures will not reduce the activity of the enzymes in this kit.

Kit Components Only

Product No.
Description
  • Endoglycosidase F1 .3 U
  • Endoglycosidase F2 .1 U
  • Endoglycosidase F3 .1 U
  • Endoglycosidase F1 reaction buffer 200 μL
  • Endoglycosidase F2 & 3 reaction buffer 200 μL

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCL9476
SLCK9717
SLCG8773
SLCF3049
SLCD4147

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity
Podzimek T, et al.
Plant Science (2018)
Junyi Ma et al.
Molecular pharmacology, 69(4), 1137-1145 (2006-01-10)
Opioid analgesics remain the choice for the treatment of moderate to severe pain. Recent research has established that the mu-opioid receptor is predominantly responsible for mediating many opioid actions, including analgesia and opioid tolerance. However, the function of delta-opioid receptors
Adela Rodríguez-Romero et al.
Acta crystallographica. Section D, Biological crystallography, 70(Pt 2), 329-341 (2014-02-18)
Endogenous glycosylated Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 constitute a potentially important IgE-binding epitope
Chaouki Benabdessalem et al.
Biochemical and biophysical research communications, 516(3), 845-850 (2019-07-03)
We previously reported that immunoreactivity of recombinant CFP32 (Rv0577), a virulence factor of Mycobacterium tuberculosis, was higher when produced in transformed Pichia pastoris as compared to transformed E. coli. In this study, we show that this difference is partly due to
M P Kolomytseva et al.
International journal of biological macromolecules, 137, 1199-1210 (2019-07-12)
Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59
View All Related Papers

Articles

O-Linked Glycan Strategies

Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Post Translational Modification

Protein modifications are crucial for disease study. Analysis methods are key.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service