Murashige and Skoog Basal Medium

Name: Murashige and Skoog Basal Medium
Brand: SIGMA

powder, suitable for plant cell culture, with Gamborg′s vitamins

Synonym(s):

MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media, MS Basal Medium

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.72

MDL number:

MFCD00240976

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Properties

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

Description

Application

Murashige and Skoog Basal Medium has been used:

to germinate the seeds for seedling growth and germination studies
in the suspension culture of Nicotiana plumbaginifolia for transformation
in the root culture of hairy clone A. artemisiifolia T4

Preparation Note

Formulated to contain 4.4 grams of powder per liter of medium.

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation