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G6392

L-Glutamine

Name: <SC>L</SC>-Glutamine
Brand: SIGMA

γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):

(S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide

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About This Item

Linear Formula:

H₂NCOCH₂CH₂CH(NH₂)CO₂H

CAS Number:

56-85-9

Molecular Weight:

146.14

MDL number:

MFCD00008044

UNSPSC Code:

12352209

eCl@ss:

32160406

PubChem Substance ID:

24895254

EC Number:

200-292-1

NACRES:

NA.75

Beilstein/REAXYS Number:

1723797

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Properties

sterility

γ-irradiated

product line

BioXtra

form

powder

packaging

vial of 0.292 g

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white to faint yellow

mp

185 °C (dec.) (lit.)

solubility

H₂O: 10 mL/vial, clear to slightly hazy

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

Description

Application

L-Glutamine has been used as a media supplement:

for cochlear organotypic culturing
in Iscove′s modified Dulbecco′s medium for HeLa cells culturing
in Dulbecco′s modified Eagle′s medium for primary hepatocyte cell culturing

Qualified for use in cell culture applications, especially serum-free cell culture.

Biochem/physiol Actions

Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.

Packaging

50 mL amber serum vial with rubber stopper.

Preparation Note

Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.

Other Notes

The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

WST-1 Assay Protocol for Cell Viability

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

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Articles

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

The signal peptide anchors apolipoprotein M in plasma lipoproteins and prevents rapid clearance of apolipoprotein M from plasma

Christoffersen C, et al.

The Journal of Biological Chemistry, 283(27), 18765-18772 (2008)

Cisplatin ototoxicity in rat cochlear organotypic cultures

Ding D, et al.

Hearing Research, 282(1-2), 196-203 (2011)

Nonresonant confocal Raman imaging of DNA and protein distribution in apoptotic cells

Uzunbajakava N, et al.

Biophysical Journal, 84(6), 3968-3981 (2003)

View All Related Papers

Global Trade Item Number

SKU	GTIN
G6392-10VL	04061833639115
G6392-1VL	04061833639122