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D9785

DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure

Synonym(s):

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

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Properties

Product Name

DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl_2,MgCl₂, MgSO_4,sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO₃: no
phenol red: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.

Preparation Note

Formulated to contain 14.8 grams of powder per liter of medium.

Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Global Trade Item Number

SKU	GTIN
D9785-10L	04061833591215