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D2906

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417
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Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

shipped in

ambient

storage temp.

2-8°C

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • Hep-2 cells
  • mouse fibroblast L‐cells
  • L‐ Wnt family member 3A (Wnt3a) cells
  • primary buffalo embryonic fibroblast (BEF)

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
088K8302
038K8319
067K83121
067K8312
085K83072

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

C Zhang et al.
Osteoarthritis and cartilage, 26(6), 824-833 (2018-03-20)
Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of
Eric M Erkenbrack et al.
PLoS biology, 16(8), e2005594-e2005594 (2018-08-25)
Among animal species, cell types vary greatly in terms of number and kind. The number of cell types found within an organism differs considerably between species, and cell type diversity is a significant contributor to differences in organismal structure and
Tao Fu et al.
FEBS letters, 593(15), 2040-2050 (2019-06-04)
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed
View All Related Papers

Global Trade Item Number

SKUGTIN
D2906-10L04061833562666
D2906-10X1L04061835518944
D2906-50K04061834178897