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B4875

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

Name: <I>N</I>&#945;-Benzoyl-<SC>DL</SC>-arginine 4-nitroanilide hydrochloride
Brand: SIGMA

trypsin substrate, chromogenic, elastase substrate, ≥98%, powder

Synonym(s):

N_α-Benzoyl-DL-arginine p-nitroanilide hydrochloride, BANI, BAPNA

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About This Item

Empirical Formula (Hill Notation):

C₁₉H₂₂N₆O₄ · HCl

CAS Number:

911-77-3

Molecular Weight:

434.88

NACRES:

NA.32

PubChem Substance ID:

24891801

UNSPSC Code:

12352204

EC Number:

213-011-2

MDL number:

MFCD00012846

Beilstein/REAXYS Number:

4081878

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Properties

Product Name

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%

assay

≥98%

form

powder

color

, white to light yellow to light brown

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H

InChI key

DEOKFPFLXFNAON-UHFFFAOYSA-N

Description

General description

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.

A chromogenic trypsin substrate.

Application

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride has been used as a substrate for trypsin and elastase in enzyme analysis.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

Global Trade Item Number

SKU	GTIN
B4875-1G	04061835504572
B4875-500MG	04061835553839
B4875-100MG	04061835540709
B4875-10G	04061835553822
B4875-5G	04061835504589