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P7582

Amino-terminal FLAG-BAP™ Fusion Protein

Synonym(s):

Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00130921
Form:
liquid
Mol wt:
~49 kDa
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form

liquid

Quality Level

200

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

General description

Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Application

Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.
Learn more product details in our FLAG® application portal.

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
045K61231
098K6107
087K6125
081K9130
105K6255

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Protocols

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀

采用M2单克隆抗体4％琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀（IP）实验方案

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Articles

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Anti-FLAG® M2磁珠

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

使用抗体 Atto 染料缀合物进行荧光多重检测

免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。

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蛋白表达

支持研究、治疗和疫苗生产的各种表达体系的蛋白质表达技术。

蛋白纯化

用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

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Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis
Eroshkin FM, et al.
Scientific Reports, 6, 23049-23049 (2016)
Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
Guo S, et al.
Biotechnology Progress, 35(1), e2722-e2722 (2019)
A Comprehensive Drosophila melanogaster Transcription Factor Interactome
Shokri L, et al.
Cell Reports, 27(3), 955-970 (2019)
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Global Trade Item Number

SKUGTIN
P7582-100UG04061834395362
P7582-25UG04061833266045
P7582-40UG04061833266052
P7582-50UG04061833266069